Journal: STAR Protocols

Article Title: Population imaging of synaptically released glutamate in mouse hippocampal slices

doi: 10.1016/j.xpro.2021.100877

Figure Lengend Snippet:

Article Snippet: Pressure application system , npi electronic , Model PDES-01AM.

Techniques: Virus, Plasmid Preparation, Recombinant, Software, Injection, Transferring, Dissection, Microscopy, Adhesive, Fluorescence, Light Microscopy, Isolation

Journal: International Journal of Molecular Sciences

Article Title: ANP and BNP Exert Anti-Inflammatory Action via NPR-1/cGMP Axis by Interfering with Canonical, Non-Canonical, and Alternative Routes of Inflammasome Activation in Human THP1 Cells

doi: 10.3390/ijms22010024

Figure Lengend Snippet: cGMP but not PKG-I is involved in non-canonical and alternative inflammasome activation. THP-1 cells were pre-treated for 10 min with ANP (10 −8 M) or BNP (10 −8 M) or cGMP (100 µM 8-Br-cGMP) in the absence or presence of LPS (10 µg/mL for 20 min) + ATP (5 mM for 40 min). When KT-5823 (1 μM) or 3-isobutyl-1-methylxanthine (IBMX) (10 μM) were used, they were added 1 h before ANP, BNP, or 8-Br-cGMP treatment. Cell lysates were immunoblotted for active Cathepsin B ( A ), Gasdermin D ( B , E ), RIPK1 ( C ), or Caspase-8 ( D , F ). Protein loading was assessed by re-probing the blots with an anti-β-actin antibody. One representative image is shown. Representative western blots images are shown. Histograms of densitometric quantification. Bars represent the ratio between respective protein and β-actin band intensity. Untreated cells were used as control and assumed as 1. All histograms indicate mean ± SD of at least n = three independent experiments, each one tested in triplicate. * p < 0.05, ** p < 0.01, *** p < 0.001 versus untreated cells; ° p < 0.05, °° p < 0.01 and °°° p < 0.001 versus LPS + ATP treated cells; # p < 0.05 versus KT-5823 + LPS + ATP treated cells.

Article Snippet: Human BNP was obtained from Phoenix Europe GmbH and dissolved in H 2 O. LPS (from Escherichia coli 0111: B4), 8-Bromoguanosine 3′,5′-cyclic monophosphate sodium salt (8-Br-cGMP), ATP (dissolved in H 2 O), and the PKG inhibitor KT-5823 and the PDEs inhibitor IBMX (3-isobutyl-1-methylxanthine) (dissolved in 100 % DMSO), were purchased from Merck KGaA (Darmstadt, Germany).

Techniques: Activation Assay, Western Blot, Control